Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/13352
Title: Melatonin attenuates the high-fat diet and streptozotocin-induced reduction in rat hippocampal neurogenesis
Authors: Wongchitrat P.
Lansubsakul N.
Kamsrijai U.
Sae-Ung K.
Mukda S.
Govitrapong P.
Keywords: beta tubulin
doublecortin
glial fibrillary acidic protein
glucose
insulin
insulin receptor
melatonin
melatonin receptor
mitogen activated protein kinase
n methyl dextro aspartic acid receptor 2A
nestin
postsynaptic density protein 95
streptozocin
synaptophysin
glutamate receptor
melatonin
streptozocin
synaptophysin
animal experiment
Article
astrocytosis
body weight
controlled study
diabetes mellitus
down regulation
enzyme phosphorylation
glucose blood level
hippocampus
hyperglycemia
immunoreactivity
lipid diet
male
memory disorder
nerve cell plasticity
nervous system development
nonhuman
priority journal
rat
signal transduction
synaptogenesis
animal
drug effects
hippocampus
hyperglycemia
memory
metabolism
nervous system development
Wistar rat
Animals
Diet, High-Fat
Hippocampus
Hyperglycemia
Male
Melatonin
Memory
Memory Disorders
Neurogenesis
Rats, Wistar
Receptors, Glutamate
Streptozocin
Synaptophysin
Issue Date: 2016
Abstract: A deviant level of melatonin in blood circulation has been associated with the development of diabetes and with learning and memory deficiencies. Melatonin might have an important function in diabetes control; however, the mechanism of melatonin in diabetes remains unknown. The present study aimed to investigate the hyperglycemic condition induced by high-fat diet (HFD) feeding and streptozotocin (STZ) injection and to examine the effect of melatonin on adult hippocampal functions. HFD-fed and STZ-treated rats significantly increased blood glucose level. The present study showed that HFD-fed and STZ-treated rats significantly impaired memory in the Morris Water Maze task, reduced neurogenesis in the hippocampus shown by a reduction in nestin, doublecortin (DCX) and β-III tubulin immunoreactivities, reduced axon terminal markers, synaptophysin, reduced dendritic marker including postsynaptic density 95 (PSD-95) and the glutamate receptor subunit NR2A. Moreover, a significant downregulation of melatonin receptor, insulin receptor-β (IR-β) and both p-IR-β and phosphorylated extracellular signal-regulated kinase (p-ERK) occurred in HFD-fed and STZ-treated rats, while the level of glial fibrillary acidic protein (GFAP) increased. Treatment of melatonin, rats had shorter escape latencies and remained in the target quadrant longer compared to the HFD-fed and STZ-treated rats. Melatonin attenuated the reduction of neurogenesis, synaptogenesis and the induction of astrogliosis. Moreover, melatonin countered the reduction of melatonin receptor, insulin receptor and downstream signaling pathway for insulin. Our data suggested that the dysfunction of insulin signaling pathway occurred in the diabetes may provide a convergent mechanism of hippocampal impaired neurogenesis and synaptogenesis lead to impair memory while melatonin reverses these effects, suggesting that melatonin may reduce the pathogenesis of diabetes. © 2016 Elsevier Ltd
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988487530&doi=10.1016%2fj.neuint.2016.09.006&partnerID=40&md5=7f3d3112813c2035f33b9080ae6b89d2
http://ir.swu.ac.th/jspui/handle/123456789/13352
ISSN: 1970186
Appears in Collections:SCOPUS 1983-2021

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