Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/13221
Title: Electrochemical detection of c-reactive protein based on anthraquinone-labeled antibody using a screen-printed graphene electrode
Authors: Jampasa S.
Siangproh W.
Laocharoensuk R.
Vilaivan T.
Chailapakul O.
Keywords: Amino acids
Antibodies
Aromatic compounds
Electrochemical electrodes
Electrochemical sensors
Graphene
Graphite electrodes
Ketones
Molecules
Voltammetry
Anthraquinone
C-reactive proteins
Differential pulse voltammetry
ELectrochemical detection
Electrochemical immunosensors
Electrochemical signals
Sandwich format
Screen-printed graphene electrodes
Chemical detection
anthraquinone derivative
antibody
C reactive protein
graphite
chemistry
electrochemical analysis
electrode
genetic procedures
human
printing
Anthraquinones
Antibodies
Biosensing Techniques
C-Reactive Protein
Electrochemical Techniques
Electrodes
Graphite
Humans
Printing
Issue Date: 2018
Abstract: In this present work, a novel electrochemical immunosensor employing a screen-printed graphene electrode (SPGE) for a simple and highly sensitive determination of C-reactive protein (CRP) in a sandwich-type format was proposed. The sensor comprised of two CRP-specific antibodies: an unlabeled capture primary antibody (Anti-1°Ab) and an electrochemically detectable anthraquinone-labeled signaling secondary (AQ-2°Ab) antibody. The Anti-1°Ab was first covalently anchored onto an L-cysteine/gold-modified disposable SPGE (L-Cys/Au/SPGE) to create the anti-CRP surface. After binding of the CRP and the AQ-2°Ab, the electrochemical signal response was measured using differential pulse voltammetry (DPV). In the presence of CRP, the sensor exhibited a significant increase in the AQ current at AQ-2°Ab compared to the negative control. The CRP concentration was detected in the range of 0.01–150 µg/mL, and the limit of detection (LOD) (S/N = 3) and limit of quantitation (LOQ) (10 SD/Slope) were 1.5 ng/mL and 10 ng/mL, respectively. This sensor exhibited very high sensitivity in determining CRP and was successfully applied to detect CRP in certified human serum with satisfactory results. The developed sensor is suitable as an alternative method for determination of CRP and the same principle may be further applied to determine other clinically important target molecules. © 2018 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042707817&doi=10.1016%2fj.talanta.2018.02.075&partnerID=40&md5=1da3da462cb55f542acd0fa03c80ed06
http://ir.swu.ac.th/jspui/handle/123456789/13221
ISSN: 399140
Appears in Collections:Scopus 1983-2021

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