Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/13102
Title: Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis
Authors: Kaewphinit T.
Ckumdee J.
Chansiri K.
Santiwatanakul S.
Keywords: Article
Au nanoprobe assay
bacterium detection
colorimetry
controlled study
diagnostic procedure
loop mediated isothermal amplification
microscopy
Mycobacterium tuberculosis
nonhuman
polymerase chain reaction
predictive value
sensitivity and specificity
sputum culture
sputum smear
tuberculosis
tuberculosis rapid test
cost
evaluation study
genetics
human
isolation and purification
molecular diagnosis
Mycobacterium tuberculosis
nanotechnology
nucleic acid amplification
procedures
time factor
tuberculosis
gold
metal nanoparticle
Colorimetry
Costs and Cost Analysis
Gold
Humans
Metal Nanoparticles
Molecular Diagnostic Techniques
Mycobacterium tuberculosis
Nanotechnology
Nucleic Acid Amplification Techniques
Sensitivity and Specificity
Time Factors
Tuberculosis
Issue Date: 2017
Abstract: Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022222151&doi=10.4103%2fijmm.IJMM_15_333&partnerID=40&md5=f3db7e86384828e5b06f548d5e592dce
http://ir.swu.ac.th/jspui/handle/123456789/13102
ISSN: 2550857
Appears in Collections:SCOPUS 1983-2021

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