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Title: A one-step rapid screening test of: Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay
Authors: Wachiralurpan S.
Sriyapai T.
Areekit S.
Sriyapai P.
Thongphueak D.
Santiwatanakul S.
Chansiri K.
Keywords: Antigen-antibody reactions
Food products
Amplification curves
Cross reaction
Limits of detection
Listeria monocytogenes
Loop mediated isothermal amplifications
Rapid screening tests
Real-time turbidimeters
Food microbiology
Issue Date: 2017
Abstract: A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL-1 and 2.82 × 103 CFU mL-1, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products. © 2017 The Royal Society of Chemistry.
ISSN: 17599660
Appears in Collections:SCOPUS 1983-2021

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