Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12716
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dc.contributor.authorChaiyo S.
dc.contributor.authorKalcher K.
dc.contributor.authorApilux A.
dc.contributor.authorChailapakul O.
dc.contributor.authorSiangproh W.
dc.date.accessioned2021-04-05T03:05:12Z-
dc.date.available2021-04-05T03:05:12Z-
dc.date.issued2018
dc.identifier.issn32654
dc.identifier.other2-s2.0-85056148744
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85056148744&doi=10.1039%2fc8an01122b&partnerID=40&md5=3729647311e9a5abdbf2b04c79b1daca
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/12716-
dc.description.abstractA novel paper-based analytical device (PAD) was fabricated and developed for the simple and rapid determination of the albumin to creatinine ratio (ACR) in urine samples. The detection was based on a colorimetric reaction using bromocresol green (BG) in a phosphate buffer (PB) at pH 4 for the determination of albumin (AL) and creatinine (CR). BG changes color from greenish-yellow to bluish-green in the presence of AL and/or CR. Picric acid (PA) in 0.25 M NaOH was used to detect CR, and PA changes color from yellow to orange. Under the optimal conditions, the working range was 10 to 350 mg dL-1 with a detection limit of 7.1 and 5.4 mg dL-1 for AL + CR and CR detection, respectively. The repeatability was evaluated, and the %RSD value was less than 8.23 (n = 10). The ACR was obtained by calculating the AL and CR colorimetric results. Finally, the proposed devices were applied to the determination of AL, CR, and ACR in urine samples. The results obtained by the developed PADs were in good agreement with the standard method and demonstrated the method could reliably measure AL, CR, and ACR. The proposed method provides a low-cost, simple, sensitive, and promising tool for diagnostic identification assay for chronic kidney disease (CKD). © 2018 The Royal Society of Chemistry.
dc.subjectbovine serum albumin
dc.subjectbromocresol green
dc.subjectcreatinine
dc.subjectpicric acid
dc.subjectanimal
dc.subjectbovine
dc.subjectchemistry
dc.subjectcolorimetry
dc.subjectdevices
dc.subjecthuman
dc.subjectlimit of detection
dc.subjectpaper
dc.subjectprocedures
dc.subjecttemperature
dc.subjectultraviolet spectrophotometry
dc.subjecturine
dc.subjectAnimals
dc.subjectBromcresol Green
dc.subjectCattle
dc.subjectColorimetry
dc.subjectCreatinine
dc.subjectHumans
dc.subjectLimit of Detection
dc.subjectPaper
dc.subjectPicrates
dc.subjectSerum Albumin, Bovine
dc.subjectSpectrophotometry, Ultraviolet
dc.subjectTemperature
dc.titleA novel paper-based colorimetry device for the determination of the albumin to creatinine ratio
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationAnalyst. Vol 143, No.22 (2018), p.5453-5460
dc.identifier.doi10.1039/c8an01122b
Appears in Collections:Scopus 1983-2021

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