Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12483
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dc.contributor.authorBoonkaew S.
dc.contributor.authorChaiyo S.
dc.contributor.authorJampasa S.
dc.contributor.authorRengpipat S.
dc.contributor.authorSiangproh W.
dc.contributor.authorChailapakul O.
dc.date.accessioned2021-04-05T03:03:39Z-
dc.date.available2021-04-05T03:03:39Z-
dc.date.issued2019
dc.identifier.issn263672
dc.identifier.other2-s2.0-85060946893
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060946893&doi=10.1007%2fs00604-019-3245-8&partnerID=40&md5=4c7683b657e9db1d32a3eb597e2665e7
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/12483-
dc.description.abstractAn origami paper-based electrochemical immunoassay for C-reactive protein (CRP) detection is described. The assay integrates multiple steps of electrode modification into a single device. A graphene-modified screen-printed carbon electrode (G/SPCE) was employed to enhance sensitivity. Gold nanoparticles were first electrodeposited onto the G/SPCE, followed by a self-assembled monolayer of L-cysteine. The capture anti-CRP was then covalently immobilized on the modified electrode. CRP was quantified by measuring the changes in the charge-transfer resistance of the electrode by using hexacyanoferrate as the redox probe. Cyclic voltammetry and scanning electron microscopy were also applied to verify the successful modification of the electrode. Under optimal conditions, impedance increase in the 0.05–100 μg mL −1 CRP concentration range, and the limit of detection is 15 ng mL −1 (at S/N = 3). The immunoassay was successfully applied to the determination of CRP in a certified human serum sample. This method is simple, low-cost, portable and disposable. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.
dc.subjectC reactive protein
dc.subjectcarbon
dc.subjectgold
dc.subjectgraphite
dc.subjectmetal nanoparticle
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectprocedures
dc.subjectC-Reactive Protein
dc.subjectCarbon
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectGold
dc.subjectGraphite
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectMetal Nanoparticles
dc.titleAn origami paper-based electrochemical immunoassay for the C-reactive protein using a screen-printed carbon electrode modified with graphene and gold nanoparticles
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationMicrochimica Acta. Vol 186, No.3 (2019)
dc.identifier.doi10.1007/s00604-019-3245-8
Appears in Collections:Scopus 1983-2021

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