Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12451
Title: N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction
Authors: Timklay W.
Magerd S.
Sato C.
Somrit M.
Watthammawut A.
Senarai T.
Weerachatyanukul W.
Kitajima K.
Asuvapongpatana S.
Keywords: concanavalin A
glycoconjugate
lectin
mannose
mannosidase
recombinant protein
thrombospondin 2
arthropod protein
glycoconjugate
thrombospondin
acrosome reaction
Article
glycosylation
histochemistry
human
immunolocalization
native polyacrylamide gel electrophoresis
nonhuman
Penaeus monodon
physiology
priority journal
protein function
sperm
Western blotting
animal
cytology
female
genetics
male
metabolism
Penaeidae
spermatozoon
Acrosome Reaction
Animals
Arthropod Proteins
Female
Glycoconjugates
Glycosylation
Male
Penaeidae
Spermatozoa
Thrombospondins
Issue Date: 2019
Abstract: Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes. Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin (pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon. A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains. The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor). The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture. In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response. Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology. © 2019 Wiley Periodicals, Inc.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061480593&doi=10.1002%2fmrd.23122&partnerID=40&md5=0210b071c7be96c10eb01a3ec4dccd90
http://ir.swu.ac.th/jspui/handle/123456789/12451
ISSN: 1040452X
Appears in Collections:Scopus 1983-2021

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