Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12409
Title: 3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications
Authors: Yakoh A.
Chaiyo S.
Siangproh W.
Chailapakul O.
Keywords: Ascorbic acid
Chemical detection
Microfluidics
Paper
ELectrochemical detection
Electrochemical sensing
Electrode modification
Fluid delivery
Label-free immunosensor
Micro-fluidic devices
Paper based devices
Voltammetric determination
Electrochemical sensors
alpha fetoprotein
gold
serotonin
chemistry
devices
electrochemistry
electrode
equipment design
immunoassay
lab on a chip
paper
alpha-Fetoproteins
Electrochemistry
Electrodes
Equipment Design
Gold
Immunoassay
Lab-On-A-Chip Devices
Paper
Serotonin
Issue Date: 2019
Abstract: This article describes the device design and fabrication of two different configurations (flow-through and stopped-flow) of a sequential fluid delivery platform on a microfluidic paper-based device. The developed device is capable of storing and transporting reagents sequentially to the detection channel without the need for external power. The device comprises two components: An origami folding paper (oPAD) and a movable reagent-stored pad (rPAD). This 3D capillary-driven device eliminates the undesirable procedure of multiple-step reagent manipulation in a complex assay. To demonstrate the scope of this approach, the device is used for electrochemical detection of biological species. Using a flow-through configuration, a self-calibration plot plus real sample analysis using a single buffer introduction are established for ascorbic acid detection. We further broaden the effectiveness of the device to a complex assay using a stopped-flow configuration. Unlike other electrochemical paper-based sensors in which the user is required to cut off the device inlet or rest for the whole channel saturation before measurement, herein a stopped-flow device is carefully designed to exclude the disturbance from the convective mass transport. As a proof of concept, multiple procedures for electrode modification and voltammetric determination of serotonin are illustrated. In addition, the research includes an impedimetric label-free immunosensor for alpha;-fetoprotein using the modified stopped-flow device. The beneficial adVantages of simplicity, low sample volume (1 mu;L), and ability to perform a complex assay qualify this innovative device for use with diverse applications. © 2019 American Chemical Society.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065083634&doi=10.1021%2facssensors.8b01574&partnerID=40&md5=ea2b5c54f238fe847ef0737b202ab2da
http://ir.swu.ac.th/jspui/handle/123456789/12409
ISSN: 23793694
Appears in Collections:Scopus 1983-2021

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