Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12366
ชื่อเรื่อง: Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii
ผู้แต่ง: Pasookhush P.
Vaniksampanna A.
Sithigorngul P.
Longyant S.
Chaivisuthangkura P.
Keywords: apoptosis
DNA
eukaryote
gene
genetic analysis
genetic structure
immune response
isolation effect
molecular analysis
protein
RNA
shrimp culture
tumor
viral disease
Arthropoda
Crustacea
Decapoda (Crustacea)
Eriocheir sinensis
Eukaryota
Fenneropenaeus chinensis
Invertebrata
Macrobrachium rosenbergii
Macrobrachium rosenbergii nodavirus
วันที่เผยแพร่: 2020
บทคัดย่อ: Translationally controlled tumor protein (TCTP) or fortilin is a highly conserved protein among eukaryotes and has important biological roles including anti-apoptotic activity which involves the elimination of virus-infected cells. In this study, the full-length cDNA of Macrobrachium rosenbergii TCTP (MrTCTP) was isolated. The cDNA sequence consisted of 783 bp with a 507-bp open reading frame encoding 168 amino acids. MrTCTP demonstrated all major characteristics of TCTP which include (1) highly homologous to TCTP protein family, (2) carrying two TCTP signatures, and (3) contained highly conserved amino acid sequences. The predicted structure of MrTCTP also revealed typical structure of TCTP which had three major domains including ß-stranded core domain, helical domain, and flexible loop. The predicted molecular mass of MrTCTP was 18.94 kDa and sequence displays 83.93 and 78.57% identity to that of Eriocheir sinensis TCTP and Fenneropenaeus chinensis TCTP, respectively. Phylogenetic analysis revealed that the deduced amino acid sequence of MrTCTP was clustered into the invertebrate branch along with the sequences from arthropods and crustaceans. The mRNA expression showed that MrTCTP expressed in every tissue examined, preferentially in hepatopancreas and muscle. In addition, MrTCTP expression in the muscle was upregulated at 1 to 4 days after infection with M. rosenbergii nodavirus (MrNV). Finally, silencing of MrTCTP increased mortality rates post-MrNV challenge. Taken together, the study shows that MrTCTP may play an important role in shrimp immune response against MrNV infection. © 2020, Springer Nature Switzerland AG.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12366
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089175354&doi=10.1007%2fs10499-020-00571-8&partnerID=40&md5=60492286895d9270ba656a7f3d247ef1
ISSN: 9676120
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.