Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12296
Title: Simultaneous and direct determination of urea and creatinine in human urine using a cost-effective flow injection system equipped with in-house contactless conductivity detector and LED colorimeter
Authors: Chaneam S.
Kaewyai K.
Mantim T.
Chaisuksant R.
Wilairat P.
Nacapricha D.
Keywords: Alkalinity
Amino acids
Ammonia
Body fluids
Cost effectiveness
Deionized water
Diodes
Enzymatic hydrolysis
Light emitting diodes
Metabolism
Sodium hydroxide
Urea
Capacitively coupled contactless conductivity detector (C4D)
Creatinine
Flow-injection analysis
Paired emitter detector diodes
Urine
Cost benefit analysis
ammonia
creatinine
glycine
politef
sodium hydroxide
urea
urease
water
creatinine
urea
accuracy
Article
dissolution
electric potential
flow injection analysis
flow rate
gas diffusion
human
hydrolysis
in house contactless conductivity detector
light emitting diode colorimeter
pH
priority journal
urinalysis
colorimetry
economics
electric conductivity
flow injection analysis
urine
Colorimetry
Creatinine
Electric Conductivity
Flow Injection Analysis
Humans
Urea
Issue Date: 2019
Abstract: This work presents a cost-effective and simple flow injection analysis (FIA) system for simultaneous and direct determination of urea and creatinine in human urine. The FIA system comprises two in-house detectors, a contactless conductivity detector and a light emitting diode (LED) detector. The contactless detector was built as a flow-through detection cell with axial electrodes, commonly known as capacitively coupled contactless conductivity detector (C4D) and the diode detector was fabricated based on the concept of paired emitter detector diodes (PEDD). With appropriate dilution of urine, the sample is directly injected into a stream of glycine-NaOH buffer pH 8.8 (the gas donor stream) and is carried by the carrier through a urease minicolumn for on-line enzymatic hydrolysis. The generated NH3 diffuses from the carrier stream through a porous polytetrafluoroethylene (PTFE) membrane into a stream of deionized water (the acceptor stream) leading to an increase in the signal at the C4D due to the NH3 dissolution in the water. In this system, creatinine is determined based on the Jaffé reaction by merging a stream of alkaline picrate with the gas donor stream. The change in color is detected using the PEDD equipped with two green LEDs. Under the optimum condition, the linear range of urea and creatinine were 30–240 mg L−1 and 10–500 mg L−1, with limits of detection of 9.0 mg L−1 and 0.9 mg L−1, respectively. The proposed system provides satisfactorily good precision (RSD < 3%), with sample throughput of 31 sample h−1 for the two analytes. The FIA system tolerates potential interference commonly found in human urine. The system was successfully applied and validated with selected reference methods. © 2019 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065554083&doi=10.1016%2fj.aca.2019.05.003&partnerID=40&md5=79b63fac48d5b3b10323df642870ba09
http://ir.swu.ac.th/jspui/handle/123456789/12296
ISSN: 32670
Appears in Collections:Scopus 1983-2021

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