Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12266
Title: Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells
Authors: Jaisin Y.
Ratanachamnong P.
Wongsawatkul O.
Watthammawut A.
Malaniyom K.
Natewong S.
Keywords: celecoxib
cyclooxygenase 2
inducible nitric oxide synthase
interleukin 6
interleukin 8
mitogen activated protein kinase p38
phosphoprotein
piperine
prostaglandin E2
stress activated protein kinase
transcription factor AP 1
1,3 benzodioxole derivative
alkaloid
amide
antiinflammatory agent
antioxidant
celecoxib
piperidine derivative
piperine
antiinflammatory activity
antioxidant activity
apoptosis
Article
cell protection
controlled study
cytotoxicity
dermatitis
enzyme linked immunosorbent assay
enzyme synthesis
HaCat cell line
human
human cell
protein expression
real time polymerase chain reaction
signal transduction
ultraviolet B radiation
Western blotting
adverse event
cell line
comparative study
dose response
drug effect
inflammation
keratinocyte
pathology
skin
ultraviolet radiation
Alkaloids
Anti-Inflammatory Agents
Antioxidants
Apoptosis
Benzodioxoles
Celecoxib
Cell Line
Dose-Response Relationship, Drug
Humans
Inflammation
Keratinocytes
Piperidines
Polyunsaturated Alkamides
Skin
Ultraviolet Rays
Issue Date: 2020
Abstract: The increase in intracellular reactive oxygen and nitrogen species plays a key role in ultraviolet B (UV-B)-induced inflammatory responses in the human skin. Piperine exhibits many pharmacological benefits. In the present study, the photoprotective effects and the possible underlying mechanisms of the anti-inflammatory effects of piperine on UV-B-irradiated keratinocytes were investigated. Piperine exerted strong, direct scavenging effects on DPPH radicals and exhibited free radical scavenging capabilities as demonstrated by the DCFH-DA and Griess assays. Consistent with these results, 10, 20, and 40 μM piperine pretreatments attenuated UV-B irradiation-induced keratinocyte cytotoxicity as reported by the resazurin assay. The highest concentration of piperine inhibited UV-B irradiation-induced cell apoptosis, as revealed by Hoechst 33342 staining. Moreover, we demonstrated the anti-inflammatory effects of piperine using western blot analysis, real-time PCR, and ELISA. Pretreatment with piperine suppressed the activation of phosphorylated p38, JNK, and AP-1 as well as the levels of COX-2/PGE2 and iNOS synthesis, while UV-B-irradiated cells triggered the induction of these signaling molecules. These results indicated that the inhibition of these inflammatory signaling pathways might play a key role in the regulation of the anti-inflammatory effects of piperine. In addition, piperine showed stronger anti-inflammatory effects than celecoxib which served as a positive control at the same concentration. All these results suggested that the anti-inflammatory properties of piperine protected keratinocytes from UV-B-induced damage, which might be due to its antioxidant properties. Therefore, piperine may be an effective therapeutic candidate compound for the treatment of UV irradiation-induced skin inflammation. © 2020 Elsevier Inc.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096213412&doi=10.1016%2fj.lfs.2020.118607&partnerID=40&md5=78ffa202704fb23198fc1df2d7a89982
http://ir.swu.ac.th/jspui/handle/123456789/12266
ISSN: 243205
Appears in Collections:Scopus 1983-2021

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