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Title: Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification
Authors: Ledlod S.
Bunroddith K.
Areekit S.
Santiwatanakul S.
Chansiri K.
Keywords: Bacilli
Bacillus cereus
Escherichia coli
Food products
Polymerase chain reaction
Gram-positive bacterium
Lactobacillus acidophilus
Lateral flow dipsticks
Listeria species
Loop mediated isothermal amplifications
Pediococcus pentosaceus
genomic DNA
protein p60
ribosephosphate pyrophosphokinase
bacterial DNA
Bacillus cereus
bacterium detection
Campylobacter coli
Campylobacter jejuni
controlled study
DNA extraction
duplex lateral flow dipstick test
Enterococcus faecalis
Escherichia coli
Lactobacillus acidophilus
Lactobacillus casei
Listeria monocytogenes
loop mediated isothermal amplification
Pediococcus pentosaceus
priority journal
Pseudomonas aeruginosa
sensitivity and specificity
Shigella dysenteriae
Shigella flexneri
Staphylococcus aureus
Vibrio cholerae
Vibrio parahaemolyticus
bacterial gene
equipment design
isolation and purification
limit of detection
Listeria monocytogenes
nucleic acid amplification
DNA, Bacterial
Equipment Design
Genes, Bacterial
Limit of Detection
Listeria monocytogenes
Meat Products
Nucleic Acid Amplification Techniques
Issue Date: 2020
Abstract: Listeria spp. are a group of gram-positive bacteria consisting of 20 species. Among them, Listeria monocytogenes is one of the major species that infects humans since it contaminates raw fruits, vegetables, and many others food products. The conventional methods for the detection of Listeria spp. and L. monocytogenes are time-consuming, taking 5–7 days. Herein, a duplex lateral flow dipstick (DLFD) test combined with loop-mediated isothermal amplification (LAMP) was developed for the identification of Listeria spp. and L. monocytogenes within approximately 45 min with the optimized LAMP reaction times at 63 °C. Under the optimized conditions, the method detection limits (MDL) with reference to genomic DNA and pure culture were 900 femtograms (fg) and 20 cfu/mL, respectively. The LAMP-DLFD showed no cross-reactivity with eighteen - other pathogenic bacteria such as Salmonella spp., Staphylococcus aureus, Escherichia coli, Campylobacter coli, C. jejuni, Enterococcus faecalis, Vibrio cholerae, V. parahaemolyticus, Pseudomonas aeruginosa, Shigella dysenteriae, S. flexneri, Bacillus cereus, Lactobacillus acidophilus, L. casei and Pediococcus pentosaceus. Among 100 samples of food products, LAMP-DLFD demonstrated 100% accuracy when compared to other standard detection methods, such as ISO11290-1, enzyme-linked fluorescent assay (ELFA) technology (VIDAS) and PCR. In conclusion, LAMP-DLFD proved to be highly specific and sensitive assays for screening detection of Listeria spp. and L. monocytogenes. © 2019 Elsevier B.V.
ISSN: 15700232
Appears in Collections:SCOPUS 1983-2021

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